Publications

[55] Pal, T.; Liu, Z.; Nair, M.G.; Chen, J.*, CRISPR in MOF formulation with enhanced stability, activity, and altered PAM specificity for broad-spectrum diagnosis of bacterial sepsis. Adanced Science (Accepted).

[54] Lin, X.; Zhai, K.; Liu, B.M.; Chen, J.*, Metabolism-driven colorimetric “read-to-answer” sensor array for bacterial discrimination and antimicrobial susceptibility testing. Analytical Chemistry 2025, 73 (29), 17040-17049.

[53] Zhao, P.; Zhou, Z.; Wolter, T.; Womelsdorf, J., Somers, A.; Feng, Y.; Nuutila, K.; Tian, Z.; Chen, J.; Tamayol, A.; Sun, W.*, Engineering microneedles for biosensing and drug delivery. Bioactive Materials 2025, 52, 36-39.

[52] Chao, A.; Wang, J.; Xiu, L.; Bold, B.; Ghonaim, A.; Chen, J.*, Hu, Q.; Yin, K., CRISPR/Cas-based biosensing strategies for non-nucleic acid contaminants in food safety: status, challenges, and perspectives. Journal of Agricultural and Food Chemistry 2025, 73 (29), 18063-18075.

[51] Pal, T.; Liu, Z.; Chen, J.*, CIMNE-CRISPR: A novel amplification-free diagnostic for rapid early detection of African Swine Fever Virus. Biosensors and Bioelectronics 2025, 273, 11715.

[50] Jiang, C.; Zhai, K.; Wright, R.C.; Chen, J.*, Engineered yeasts displaying PETase and MHETase as whole-cell biocatalysts for the degradation of polyethylene terephthalate (PET). ACS Synthetic Biology 2025, 14, 7, 2910-2820.

[49] He, Y.; Chen, J.*, CRISPR/Cas9-mediated genome editing of T4 bacteriophage for high-throughput antimicrobial susceptibility testing. Analytical Chemistry 2024. DOI:10.1021/acs.analchem.4c05177.

[48] Liu, L.; Kasputis, T.; Chen, J.; Moore, M.D.; Du, K.*, Fully integrated microfluidic digital chip for simple and highly quantitative detection of norovirus. Analytical Chemistry 2024. DOI:10.1021/acs.analchem.4c03152.

[47] Zhao, X.; He, Y.; Shao, S.; Ci, Q.; Chen, L.; Lu, X.; Liu, Q.; Chen, J.*, CRISPR/Cas14 and G-Quadruplex DNAzyme-driven biosensor for paper-based colorimetric detection of African swine fever virus. ACS Sensors 2024, 9 (5), 2413-2420.

[46] Kasputis, T.; Yeh, P.-C.; Liu, L.; Marano, J.; Weger-Lucarelli, J.; Du, K.; Lin, L.; Chen, J.*, Development of a self-powered digital LAMP microfluidic chip (SP-dChip) for the detection of emerging viruses. Lab on a Chip 2024, 24 (14), 3490-3497.

[45] Kasputis, T.; Hosmer, K. E.; He, Y.; Chen, J.*, Ensuring food safety: microfluidic-based approaches for the detection of food contaminants. Analytical Science Advances 2024, e202400003.

[44] Kasputis, T.; He, Y.; Ci, Q.; Chen, J.*, On-site fluorescent detection of sepsis-inducing bacteria using a graphene-oxide CRISPR-Cas12a (GO-CRISPR) system. Analytical Chemistry 2024, 96 (6), 2676-2683.

[43] Duan, M.; Zhao, Y.; Liu, Y.; He, Y.; Dai, R.; Chen, J.; Li, X.; Jia, F.*, A low-background and wash-free signal amplification F-CRISPR biosensor for sensitive quantitative and visible qualitative detection of Salmonella Typhimurium. Science of The Total Environment 2024, 912, 168905.

[42] Duan, M.; Li, B.; He, Y.; Zhao, Y.; Liu, Y.; Zou, B.; Liu, Y.; Chen, J.; Dai, R.; Li, X.*, A CG@ MXene nanocomposite-driven E-CRISPR biosensor for the rapid and sensitive detection of Salmonella Typhimurium in food. Talanta 2024, 266, 125011.

[41] Deng, R.; Xu, L.; Zhang, Y.; Zhang, X.; Yuan, Z.; Chen, J.*; Xia, X.*, CRISPR-based nucleic acid assays for food authentication. Trends in Food Science & Technology 2024, 145, 104351.

[40] Ci, Q.; He, Y.; Chen, J.*, Novel anti-CRISPR-assisted CRISPR biosensor for exclusive detection of single-stranded DNA (ssDNA). ACS Sensors 2024, 9 (3), 1162-1167.

[39] Zhao, X.; Rahman, M.; Xu, Z.; Kasputis, T.; He, Y.; Yuan, L.; Wright, R. C.; Chen, J.*, Engineered yeast displaying specific norovirus-binding nanobodies for the concentration and detection of human norovirus in food matrix. Journal of Agricultural and Food Chemistry 2023, 71 (22), 8665-8672.

[38] Jia, F.; Li, B.; He, Y.; Shen, Y.; Chen, J.; Li, X.; Li, Y.*, An amplification-free CRISPR-SERS biosensor for specific, sensitive and rapid detection of Salmonella Typhimurium in poultry. LWT 2023, 189, 115476.

[37] Huang, D.; Yuan, M. M.; Chen, J.; Zheng, X.; Wong, D.; Alvarez, P. J.; Yu, P.*, The association of prokaryotic antiviral systems and symbiotic phage communities in drinking water microbiomes. ISME Communications 2023, 3 (1), 46.

[36] He, Y.; Xu, Z.; Kasputis, T.; Zhao, X.; Ibañez, I.; Pavan, F.; Bok, M.; Malito, J. P.; Parreno, V.; Yuan, L.; Wright, R. C.; Chen, J.*, Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV-2. ACS Applied Materials & Interfaces 2023, 15 (31), 37184-37192.

[35] He, Y.; Shao, S.; Chen, J.*, High-fidelity identification of single nucleotide polymorphism by Type V CRISPR systems. ACS Sensors 2023, 8 (12), 4478-4483.

[34] He, Y.; Hu, Q.; San, S.; Kasputis, T.; Splinter, M. G. D.; Yin, K.; Chen, J.*, CRISPR-based biosensors for human health: A novel strategy to detect emerging infectious diseases. TrAC Trends in Analytical Chemistry 2023, 117342.

[33] Bao, M.; Waitkus, J.; Liu, L.; Chang, Y.; Xu, Z.; Qin, P.; Chen, J.; Du, K.*, Micro- and nanosystems for the detection of hemorrhagic fever viruses. Lab on a Chip 2023, 23 (19), 4173-4200.

[32] He, J.; Peng, R.; Yuqing, H.; Karim, R.; Chen, J.; Lu, G.; Du, K.*, Superhydrophobic rotation-chip for computer-vision identification of drug-resistant bacteria. ACS Applied Materials & Interfaces 2023, 15 (23), 27732-27741.

[31] Ci, Q.; Yang, H.; Bian, Y.; Li, Z.; Liu, J.; Wei, J.; Yang, N.; Chen, J.; Li, L.; Zhang, C.*, An in-situ fluorescent copolymer dots-based kit for the specific detection of monoamine oxidase-A in cell/tissue/human prostate cancer. Sensors and Actuators B: Chemical 2023, 397, 134655.

[30] Yu, Z.; Jung, D.; Park, S.; Hu, Y.; Huang, K.; Rasco, B. A.; Wang, S.; Ronholm, J.; Lu, X.; Chen, J.*, Smart traceability for food safety. Critical Reviews in Food Science and Nutrition 2022, 62 (4), 905-916.

[29] Yu, Z.; Chen, J.; Tan, Y.; Shen, Y.; Zhu, L.; Yu, P.*, Phage predation promotes filamentous bacterium piscinibacter colonization and improves structural and hydraulic stability of microbial aggregates. Environmental Science & Technology 2022, 56 (22), 16230-16239.

[28] Kasputis, T.; Hilaire, S. S.; Xia, K.; Chen, J.*, Colorimetric detection of antimicrobial resistance from food processing facilities using a CRISPR system. ACS Food Science & Technology 2022, 3 (1), 17-22.

[27] He, Y.; Jia, F.; Sun, Y.; Fang, W.; Li, Y.; Chen, J.*; Fu, Y.*, An electrochemical sensing method based on CRISPR/Cas12a system and hairpin DNA probe for rapid and sensitive detection of Salmonella Typhimurium. Sensors and Actuators B: Chemical 2022, 369, 132301.

[26] Gu, X.; Huang, D.; Chen, J.; Li, X.; Zhou, Y.; Huang, M.; Liu, Y.; Yu, P.*, Bacterial inactivation and biofilm disruption through indigenous prophage activation using low-intensity cold atmospheric plasma. Environmental Science & Technology 2022, 56 (12), 8920-8931.

[25] Zhang, X.; Wu, Y.; Chen, J.; Yang, Y.; Li, G.*, Bioinspired artificial “clickase” for the catalytic click immunoassay of foodborne pathogens. Analytical Chemistry 2021, 93 (6), 3217-3225.

[24] Tao, M.; Chen, J.; Huang, K.*, Bio-based antimicrobial delivery systems for improving microbial safety and quality of raw or minimally processed foods. Current Opinion in Food Science 2021, 41, 189-200.

[23] Chen, A.; Wang, D.; Nugen, S. R.*; Chen, J.*, An engineered reporter phage for the fluorometric detection of Escherichia coli in ground beef. Microorganisms 2021, 9 (2), 436.

[21] Chen, J.; Liu, C.-Y.; Wang, X.; Sweet, E.; Liu, N.; Gong, X.; Lin, L.*, 3D printed microfluidic devices for circulating tumor cells (CTCs) isolation. Biosensors and Bioelectronics 2020, 150, 111900.

[20] Chen, J.; Jiang, F.; Huang, C.-W.; Lin, L.*, Rapid genotypic antibiotic susceptibility test using CRISPR-Cas12a for urinary tract infection.

Analyst 2020, 145 (15), 5226-5231.

Before Independence

2015-2019

[19] Zang, X.; Jian, C.; Zhu, T.; Fan, Z.; Wang, W.; Wei, M.; Li, B.; Follmar Diaz, M.; Ashby, P.; Lu, Z.; Chu, Y.; Wang, Z.; Ding, X.; Xie, Y.; Chen, J.; Hohman, N. J.; Sanghadasa, M.; Grossman, J. C.; Lin, L.*, Laser-sculptured ultrathin transition metal carbide layers for energy storage and energy harvesting applications. Nature Communications 2019, 10 (1), 3112.

[18] Wang, D.; Hinkley, T.; Chen, J.; Talbert, J. N.; Nugen, S. R.*, Phage-based electrochemical detection of Escherichia coli in drinking water using affinity reporter probes. Analyst 2019, 144 (4), 1345-1352.

[17] Chen, J.; Nugen, S. R.*, Detection of protease and engineered phage-infected bacteria using peptide-graphene oxide nanosensors. Analytical and Bioanalytical Chemistry 2019, 411, 2487-2492.

[16] Wang, D.; Chen, J.; Nugen, S. R.*, Electrochemical detection of Escherichia coli from aqueous samples using engineered phages. Analytical Chemistry 2017, 89 (3), 1650-1657.

[15] Dai, M.; Chen, J.; Goddard, J. M.; Nugen, S. R.*, Facile hierarchical assembly of gold particle decorated conductive polymer nanofibers for electrochemical sensing. Nanotechnology 2017, 28 (6), 06LT01.

[14] Chen, J.; Picard, R. A.; Wang, D.; Nugen, S. R.*, Lyophilized engineered phages for Escherichia coli detection in food matrices. ACS Sensors 2017, 2 (11), 1573-1577.

[13] Chen, J.; Andler, S. M.; Goddard, J. M.; Nugen, S. R.*; Rotello, V. M.*, Integrating recognition elements with nanomaterials for bacteria sensing. Chemical Society Reviews 2017, 46 (5), 1272-1283.

[12] Chen, J.; Alcaine, S. D.; Jackson, A. A.; Rotello, V. M.*; Nugen, S. R.*, Development of engineered bacteriophages for Escherichia coli detection and high-throughput antibiotic resistance determination. ACS Sensors 2017, 2 (4), 484-489.

[11] Wang, Z.; Wang, D.; Chen, J.; Sela, D. A.; Nugen, S. R.*, Development of a novel bacteriophage-based biomagnetic separation method as an aid for sensitive detection of viable Escherichia coli. Analyst 2016, 141 (3), 1009-1016.

[10] Wang, P.; Pang, S.; Chen, J.; McLandsborough, L.; Nugen, S. R.*; Fan, M.; He, L., Label-free mapping of single bacterial cells using surface-enhanced Raman spectroscopy. Analyst 2016, 141 (4), 1356-1362.

[9] Wang, D.; Wang, Z.; Chen, J.; Kinchla, A. J.; Nugen, S. R.*, Rapid detection of Salmonella using a redox cycling-based electrochemical method. Food Control 2016, 62, 81-88.

[8] Huang, K.; Chen, J.; Nugen, S. R.; Goddard, J. M.*, Hybrid antifouling and antimicrobial coatings prepared by electroless co-deposition of fluoropolymer and cationic silica nanoparticles on stainless steel: efficacy against Listeria monocytogenes. ACS Applied Materials & Interfaces 2016, 8 (25), 15926-15936.

[7] Chen, J.; Pang, S.; He, L.; Nugen, S. R.*, Highly sensitive and selective detection of nitrite ions using Fe3O4@ SiO2/Au magnetic nanoparticles by surface-enhanced Raman spectroscopy. Biosensors and Bioelectronics 2016, 85, 726-733.

[6] Chen, J.; Jackson, A. A.; Rotello, V. M.; Nugen, S. R.*, Colorimetric detection of Escherichia coli based on the enzyme-induced metallization of gold nanorods. Small 2016, 12 (18), 2469-2475.

[5] Chen, J.; Zhou, Y.; Wang, D.; He, F.; Rotello, V. M.; Carter, K. R.; Watkins, J. J.; Nugen, S. R.*, UV-nanoimprint lithography as a tool to develop flexible microfluidic devices for electrochemical detection. Lab on a Chip 2015, 15 (14), 3086-3094.

[4] Chen, J.; Li, Y.; Huang, K.; Wang, P.; He, L.; Carter, K. R.; Nugen, S. R.*, Nanoimprinted patterned pillar substrates for surface-enhanced Raman scattering applications. ACS Applied Materials & Interfaces 2015, 7 (39), 22106-22113.

[3] Chen, J.; Jiang, Z.; Ackerman, J. D.; Yazdani, M.; Hou, S.; Nugen, S. R.*; Rotello, V. M.*, Electrochemical nanoparticle-enzyme sensors for screening bacterial contamination in drinking water. Analyst 2015, 140 (15), 4991-4996.

[2] Chen, J.; Duncan, B.; Wang, Z.; Wang, L.-S.; Rotello, V. M.*; Nugen, S. R.*, Bacteriophage-based nanoprobes for rapid bacteria separation. Nanoscale 2015, 7 (39), 16230-16236.

[1] Chen, J.; Alcaine, S. D.; Jiang, Z.; Rotello, V. M.; Nugen, S. R.*, Detection of Escherichia coli in drinking water using T7 bacteriophage-conjugated magnetic probe. Analytical chemistry 2015, 87 (17), 8977-8984.

Chen Research Group in Bioengineering

Publications

Before Independence

Bourns College of Engineering